Pathology and Laboratory Medicine
- (608) 263-0058
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Aligned research focus:
Regenerative medicine, stem cells
Organ system/disease focus:
Hematopoietic and vascular system/ blood cancer therapies
The main focus of research in my lab is to significantly advance the clinical use of stem cells through development of novel sources of hematopoietic stem cells, mature blood cells, mesenchymal and endothelial progenitors for transplantation, transfusion and cancer immunotherapy. We use integrative approaches, including genomics, proteomics and bioinformatics, to achieve the outlined goals and to understand important cellular and molecular events leading to mesoderm and blood cell development and diversification. We already developed a very efficient system for hematopoietic and endothelial differentiation of hES and iPS cells, and directed differentiation of hES cells toward red blood cells, dendritic cells, macrophages, osteoclasts and granulocytes. In addition, we defined the major cellular pathways leading to formation of blood cells and identified several novel hematoendothelial, hematopoietic and mesenchymal progenitors. Through comparative analysis of transcriptome and engraftment properties of these novel progenitors and fetal primitive blood cells as well as employing loss-of- and gain-on-function and lineage-tracing experiments, we expect to gain fundamental insights into molecular mechanisms leading to blood and endothelial cell development. These studies could ultimately revolutionize cellular therapies for blood cancer, hereditary blood and cardiovascular disease.
Choi KD, Vodyanik MA, Slukvin II. Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell–derived lin-CD34+CD43+CD45+ progenitors. Journal of Clinical Investigation. 2009;119(9):2818-29.
Vodyanik MA, Yu J, Zhang X, Tian S, Stewart R, Thomson JA, Slukvin II. A mesoderm-derived precursor for mesenchymal stem and endothelial cells. Cell Stem Cell 2010 Dec 3;7(6):718-29.
Hu K, Yu J, Suknuntha K, Tian S, Montgomery K, Choi KD, Stewart R, Thomson JA, Slukvin II. Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells. Blood. 2011 Feb 4. [Epub ahead of print]