Comparative Biosciences, Obstetrics and Gynecology
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Aligned research focus:
Women’s reproductive health, maternal-fetal medicine, reproductive endocrinology and infertility
Organ system/disease focus:
Recurrent pregnancy loss, female reproductive tract immunology, embryo implantation and placental biology, intrauterine/prenatal programming
The Golos laboratory examines questions of placental biology relevant to human health and disease, using nonhuman primate models, human clinical materials, and human and nonhuman primate embryonic stem cells in its studies. Study of the maternal-fetal immune dialogue examines nonpolymorphic MHC class I molecules expressed on placental cells and their interactions with the maternal immune system, particularly endometrial natural killer cells and macrophages in promoting pregnancy success, including placental and decidual vascularization. In addition, embryonic stem cells and primate embryos are used to model implantation and placental morphogenesis, with 2-dimensional and 3-dimensional modeling and co-culture systems for understanding how placental cells carry on a dialog with the maternal endometrium. At the interface of these projects is the effect of maternal immune cells directly on rhesus blastocyst function and growth in 3-dimensional co-culture.
Maternal immune recognition of pregnancy and regulation of a healthy implantation site and placenta may play a significant role in pregnancy issues ranging from miscarriage and recurrent pregnancy loss to preeclampsia and intrauterine growth restriction. The primate model will allow direct experimentation at the maternal-fetal interface to develop experimental and therapeutic models of utero-placental function and dysfunction.
Bondarenko G.I., Burleigh D.W., Durning M., Breburda E.E., Grendell R.L., and T.G. Golos. 2007. Passive Immunization against the MHC Class I Molecule Mamu-AG Disrupts Rhesus Placental Development and Endometrial Responses. J. Immunol. 179:8042-8050.
Douglas, G.C., C.A. VandeVoort, P. Kumar, T.C. Chang, and T.G. Golos, 2009. Trophoblast stem cells: Models for Investigating Trophectoderm Differentiation and Placental Development. Endocrine Reviews. 30:228-40. PMCID: PMC2726840
Golos, T.G., Bondarenko G.I., Dambaeva, S.V., Breburda, E.E., and M. Durning. 2010. On the role of placental major histocompatibility complex and decidual leukocytes in implantation and pregnancy success using non-human primate models. Int. J. Dev. Biol. 54:431-443.