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University of Wisconsin–Madison
Wisconsin National Primate Research Center
A legacy of life-saving research and humane animal care

Assay Services Unit

Assay Services lab members

Assay Services provides investigators with modern, efficient, and cost-effective endocrine and biomarker measurements in a centralized facility. We provide our services to Primate Center Investigators, members of the UW Institute of Clinical and Translational Research (ICTR), other CTSA members (Clinical and Translational Science Awards), and NPRCs, as well as investigators at other universities across the United States and internationally. Our focus is to provide method development and high throughput analyses of steroids, proteins, peptides, bioamines, biomarkers, and drug compounds to aid NIH-funded research with an emphasis on non-human and human primates.

CategoriesDescription and mediaPre sample TreatmentMethods of measurement
cytokinessingle analytes: serum, plasma, urinedirectEIA
peptidessingle analytes: serum, plasma, CSF, urine, saliva, tissue, cell culture, brain perfusionssolid phase extraction,
direct
EIA, RIA, LC/ECD for bioamines
proteinssingle analytes: serum, plasma, CSF, urine, cell culture, saliva, tissue, brain perfusionssolid phase extraction,
direct
EIA, RIA, protein determination
steroidssingle and multisteroid:
serum, plasma, urine, feces, saliva, CSF, tissue, cell culture, brain perfusions, hair
liquid-liquid extractions, HPLC, solid phase extraction, celite chromatographyLC/MS/MS,
EIA, RIA

These are some examples our of prices of the 80+ analytes that Assays Services offers. Please contact us with prices and any other questions you might have. Due to sample prep, instruments used, analytes and other costs we can’t display a full list of prices. Our goal is to work with our customer and their needs in order to find the best price per sample for the methodology and instruments used.

Analyte(s) Sample typeAssay MethodCost per sample
catecholamines urine or plasmaLC/ECD$60.29
cortisolsalivaRIA$15.87
follicle stimulating hormone (FSH)serumRIA$14.99
kisspeptin serumRIA$13.54
luteinizing hormone (LH)serumRIA$14.00
multisteroid panel - 6 (cortisol, progesterone, testosterone, androstenedione, estradiol, estrone)serumLC/MS/MS$111.05
oxytocinurineEIA$26.80
progesteroneplasmaEIA$17.69
vitamin D panel (cholecalciferol, 25 hydroxyvitamin D2&3, 24,25 dihydroxyvitamin D2&3)serumLC/MS/MS$111.05

EIA – enzymeimmunoassay, RIA – radioimmunoassay, LC/MS/MS – liquid chromatography/tandem mass spectrometry, LC/ECD – liquid chromatography/eletrochemical detection

Key Personnel

NAME POSITION PHONE E-MAIL
Toni Ziegler, PhD Senior Scientist & Director Assay Services (608) 263-3507 Email Dr. Ziegler
Amita Kapoor, PhD Assistant Scientist (608) 263-3519 Email Dr. Kapoor
Hemanta Shrestha, PhD Senior Research Specialist (608) 263-5667 Email Dr. Shrestha
Cody James M. Corbett Research Specialist (608) 263-3519 Email Cody Corbett

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