Immunology Services (IS) is a research service unit of the Wisconsin National Primate Research Center, that specializes in measuring antigen specific cellular responses of nonhuman primates. IS performs standardized elispot, and flow cytometry-based immune assays, and conducts vaccination experiments. In addition to routine tests IS develops custom immune-assays to meet the specific aims of collaborating investigators. Primate Center investigators and other NIH supported researchers receive priority attention from IS, but anyone is welcome to contact us.
- Antigen-specific Elispot assays.
- Antigen-specific intracellular cytokine assays (ICS).
- Multicolor flow cytometric phenotype analysis (up to 17 parameters).
- BL-3 level cell sorting by multicolor flow cytometry (up to 8 parameters).
- Monoclonal antibody production from supernatants of hybridomas.
- Fluorochrome conjugation to purified monoclonal antibodies.
- SIV or HIV-specific ADCC assays.
- Vaccination experiments in nonhuman primates.
|Flow cytometry facility usage||Kim Weisgrau||Senior Research Specialist||Send email to Kim Weisgrau|
|Fluorochrome-conjugated antibodies||Logan Vosler||Associate Research Specialist||Send email to Logan Vosler|
|SIV or HIV-specific ADCC assays||David Evans||Professor||Send email to Dr. Evans|
|Vaccination experiments, antigen-specific and custom immune assays||Eva Rakasz||Senior Scientist||Send email to Dr. Rakasz|
- Fluorofinder sign in
- Introduction to Flow Cytometry Web-Based Training I
- Introduction to Flow Cytometry Web-Based Training II
- Stain index
Standardized Flow cytometry staining panels
Immunology Services has standardized staining panels to monitor the phenotype and functional capability of various T cell subsets, including memory and naive T cell populations, follicular helper T cells, and regulatory T cells. We also quantify plasmablast cells, myeloid and plasmacytoid dendritic cells, classical, non-classical and intermediate monocytes. Our standardized assays to measure the frequency and functional capacity of various NK cells subsets have been published in Cytometry A (see links below).
- Activation Panel for Rhesus Macaque NK Cell Subsets
- Functional Analysis of Natural Killer Cell Subsets in Macaques
- Ex Vivo Analysis of SIV Infected Cells by Flow Cytometry