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University of Wisconsin–Madison
Wisconsin National Primate Research Center
A legacy of life-saving research and humane animal care

Immunology Services

WNPRC Immunology Services staff member Kim Weisgrau acquires immunophenotype data on the BD FACSymphony flow cytometer.

Overview

Immunology Services (IS) is a research service unit of the Wisconsin National Primate Research Center that specializes in measuring antigen-specific cellular responses in nonhuman primates (NHPs) and antibody-dependent cell-mediated cytotoxicity (ADCC) in NHPs and humans.

IS performs standardized ELISpot and flow cytometry-based immune assays, and conducts vaccination experiments. In addition to routine tests, IS offers consultation on the design of flow cytometry experiments and develops custom immune-assays to meet the specific aims of collaborating investigators. Primate Center investigators and other NIH-supported researchers receive priority attention from IS, but anyone is welcome to contact us. Learn how we support investigators at Immunology Services Case Studies.

We provide the following services:

  • ELISpot assays to measure antigen-specific T cell and plasmablast responses.
  • Intracellular Cytokine assays to measure antigen-specific T cell responses.
  • Multicolor flow cytometric phenotype analysis (up to 17 parameters).
  • BSL3 level-cell sorting by multicolor flow cytometry (up to 8 parameters).
  • Monoclonal antibody production from supernatants of hybridomas.
  • Fluorochrome conjugation to purified monoclonal antibodies.
  • SIV or HIV-specific ADCC assays.
  • Vaccination experiments in nonhuman primates.

Standardized Flow Cytometry Staining Panels

Immunology Services has standardized staining panels to monitor the phenotype and functional capability of various T cell subsets, including memory and naive T cell populations, follicular helper T cells, and regulatory T cells. We also quantify plasmablast cells, myeloid and plasmacytoid dendritic cells, classical, non-classical and intermediate monocytes. Our standardized assays to measure the frequency and functional capacity of various NK cells subsets have been published in Cytometry A (see links below).

We developed a staining panel to identify and quantify neutrophil progenitors in rhesus macaques.

To request service, please contact the appropriate Immunology Services staff member, below.

Position Name E-mail
Immunology Services Unit Head Dr. Eva Rakasz Contact Dr. Rakasz
ADCC Assays Dr. David T. Evans Contact Dr. Evans
Flow-cytometry Facility Usage Kim Weisgrau Contact Kim Weisgrau
Fluorochrome-conjugated antibodies Michael Grunst Contact Michael Grunst

WNPRC Immunology Services Price List (pdf)

WNPRC Immunology Services publications

Related Links

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