University of Wisconsin–Madison

Immunology Services

WNPRC Immunology Services members are, from the left, Kim Weisgrau, Unit Head Eva Rakasz, Amanda Johnson, Logan Vosler and Glen Grandea. (J. Lenon image)

Overview

Immunology Services (IS) is a research service unit of the Wisconsin National Primate Research Center, that specializes in measuring antigen specific cellular responses of nonhuman primates. IS performs standardized elispot, and flow cytometry-based immune assays, and conducts vaccination experiments. In addition to routine tests IS develops custom immune-assays to meet the specific aims of collaborating investigators. Primate Center investigators and other NIH supported researchers receive priority attention from IS, but anyone is welcome to contact us.

Services Supported

  • Antigen-specific Elispot assays.
  • Antigen-specific intracellular cytokine assays (ICS).
  • Multicolor flow cytometric phenotype analysis (up to 17 parameters).
  • BL-3 level cell sorting by multicolor flow cytometry (up to 8 parameters).
  • Monoclonal antibody production from supernatants of hybridomas.
  • Fluorochrome conjugation to purified monoclonal antibodies.
  • SIV or HIV-specific ADCC assays.
  • Vaccination experiments in nonhuman primates.

Contact information

Flow cytometry facility usage Kim Weisgrau Senior Research Specialist Send email to Kim Weisgrau
Fluorochrome-conjugated antibodies Logan Vosler Associate Research Specialist Send email to Logan Vosler
SIV or HIV-specific ADCC assays David Evans Professor Send email to Dr. Evans
Vaccination experiments, antigen-specific and custom immune assays Eva Rakasz Senior Scientist Send email to Dr. Rakasz

Related Links

Standardized Flow cytometry staining panels

Immunology Services has standardized staining panels to monitor the phenotype and functional capability of various T cell subsets, including memory and naive T cell populations, follicular helper T cells, and regulatory T cells. We also quantify plasmablast cells, myeloid and plasmacytoid dendritic cells, classical, non-classical and intermediate monocytes. Our standardized assays to measure the frequency and functional capacity of various NK cells subsets have been published in Cytometry A (see links below).

Pricing Information