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University of Wisconsin–Madison
Wisconsin National Primate Research Center
A legacy of life-saving research and humane animal care

Immunology Services

WNPRC Immunology Services staff member Logan Vosler counts cells on the BD LSRII flow cytometer.

WNPRC Immunology Services staff member Logan Vosler acquires immunophenotype data on the BD LSRII flow cytometer.

Overview

Immunology Services (IS) is a research service unit of the Wisconsin National Primate Research Center that specializes in measuring antigen-specific cellular responses in nonhuman primates (NHPs) and antibody-dependent cell-mediated cytotoxicity (ADCC) in NHPs and humans.

IS performs standardized ELISpot and flow cytometry-based immune assays, and conducts vaccination experiments. In addition to routine tests, IS develops custom immune-assays to meet the specific aims of collaborating investigators. Primate Center investigators and other NIH-supported researchers receive priority attention from IS, but anyone is welcome to contact us.

We provide the following services:

  • ELISpot assays to measure antigen-specific T cell and plasmablast responses.
  • Intracellular Cytokine assays to measure antigen-specific T cell responses.
  • Multicolor flow cytometric phenotype analysis (up to 17 parameters).
  • BSL3 level-cell sorting by multicolor flow cytometry (up to 8 parameters).
  • Monoclonal antibody production from supernatants of hybridomas.
  • Fluorochrome conjugation to purified monoclonal antibodies.
  • SIV or HIV-specific ADCC assays.
  • Vaccination experiments in nonhuman primates.

STANDARDIZED FLOW CYTOMETRY STAINING PANELS

Immunology Services has standardized staining panels to monitor the phenotype and functional capability of various T cell subsets, including memory and naive T cell populations, follicular helper T cells, and regulatory T cells. We also quantify plasmablast cells, myeloid and plasmacytoid dendritic cells, classical, non-classical and intermediate monocytes. Our standardized assays to measure the frequency and functional capacity of various NK cells subsets have been published in Cytometry A (see links below).

We developed a staining panel to identify and quantify neutrophil progenitors in rhesus macaques.

To request service, please contact the appropriate Immunology Services staff member, below.

Immunology Services Unit Head: Dr. Eva Rakasz Contact Dr. Rakasz.

ADCC Assays: Dr. David T. Evans Contact Dr. Evans
Flow-cytometry Facility Usage: Kim Weisgrau Contact Kim Weisgrau
Fluorochrome-conjugated antibodies: Logan Vosler Contact Logan Vosler

WNPRC Immunology Services Price List

WNPRC Immunology Services publications

RELATED LINKS

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