How do I request a service?
Please contact Genetics Services. We will assist you with choosing an assay, provide precise total costs for your project, and help you through the process of setting up a service contract with the WNPRC.
How do I submit samples for MHC genotyping?
For all MHC assays, please submit an electronic copy (Excel file) of your sample IDs to the Genetics Services MHC group email prior to shipping samples, providing an intended date for shipment. Upon shipment, please provide a tracking number; any major carrier can be used. MHC assay shipments should be addressed to:
AIDS Vaccine Research Laboratory, Attn: Roger Wiseman/Trent Prall, 555 Science Dr, Madison, WI 53711, 608-890-0847
- For Mauritian cynomolgus or Indian rhesus MiSeq genotyping, genomic DNA samples can be shipped overnight at room temperature, fresh blood samples can be shipped overnight on cold packs, and frozen blood and cryopreserved cell samples should be shipped overnight on dry ice.
- For other nonhuman primate MiSeq genotyping or PacBio full-length transcript analysis, cDNA samples may be shipped overnight at room temperature, RNA and cell samples should be shipped overnight on dry ice (avoiding freeze-thaw cycles), and fresh whole blood samples should be shipped overnight on cold packs.
How do I submit samples for pathogen sequencing?
For all pathogen sequencing assays, please contact the Genetics Services pathogen sequencing group prior to shipping samples, providing an intended date for shipment. Upon shipment, please provide a tracking number; any major carrier can be used. Pathogen sequencing shipments should be addressed to:
AIDS Vaccine Research Laboratory, Attn: Shelby O’Connor, 555 Science Dr, Madison, WI 53711, 608-890-0843
- Plasma samples should be shipped overnight on dry ice. Best results are obtained if the plasma was promptly stored at -80ºC upon isolation and never thawed. Self-isolated RNA or DNA should also be shipped overnight on dry ice, and the shipment should include concentration information for each sample.
How long does it typically take to receive results?
All samples are processed on a first-come, first-served basis.
For MHC genotyping assays on the MiSeq, results are typically returned within 8-12 weeks of receipt of sample. Expedited service can also be requested for a 50% premium on base price; results for expedited service would be available in 4-6 weeks.
For PacBio MHC genotyping assays and viral genome characterization, we are unable to offer expedited service at this time. PacBio MHC genotyping assay results are typically available in 12-16 weeks from receipt of sample.
An estimate of time to receive pathogen sequencing results will be provided upon receipt of samples.
What is the minimum viral load necessary for SIV sequencing?
SIV sequencing is most reliable when there are at least 1,000 viral RNA templates in the sample.