University of Wisconsin–Madison

Virology Services Unit

WNPRC Virology Services includes, from the left, Andrea Weiler, Gabrielle Lehrer-Brey and Unit Head Tom Friedrich. (J. Lenon image)

The WNPRC Virology Services Unit provides expert support to investigators performing virological research in nonhuman primates. Our services include highly sensitive molecular diagnostics, production and titration of virus stocks suitable for in-vitro and/or in-vivo studies, generation of custom virus mutants by reverse genetics, virus isolation and more.

Our clients include investigators studying the AIDS viruses SIV and SHIV, influenza, Zika virus, and dengue, among other viruses.

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Services Supported

  • Detection of viral RNA by ultrasensitive quantitative RT-PCR (QRT-PCR). Viral targets include:
    • SIV/SHIV
    • Simultaneous quantification of wild type SIV and nef deletion mutants
    • Influenza viruses
    • Zika virus
    • Dengue virus
    • Yellow fever virus 17D
    • Simian arteriviruses
  • Production of characterized virus stocks:
    • Viruses can be purchased from existing stocks on a per-vial basis
    • We can also produce large-scale stocks to order
    • Information on Zika virus stock PRVABC59, isolated from Puerto Rico
    • Please contact us for details
  • Production of custom SIV mutants
  • Isolation of NHP viruses in tissue culture
  • Assays to measure the latent reservoir of SIV:
    • Quantitative viral outgrowth assay
    • Quantification of cell-associated SIV DNA by droplet digital PCR (ddPCR) or QPCR

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